Diagnostic Histopathology
Volume 15, Issue 3 , Pages 142-150, March 2009

How to make tissue microarrays

Mike Parsons MB BCh BSc is a Specialty Registrar in Histopathology at the Department of Histopathology and Molecular Pathology, University of Leeds, St James University Hospital, Leeds, UK

Heike Grabsch MD is a Senior Clinical Lecturer in Gastrointestinal Pathology and Honorary Consultant Histopathologist at the Leeds Institute of Molecular Medicine, University of Leeds, St James University Hospital, Leeds, UK

published online 24 February 2009.

Abstract 

It has been 10 years since tissue microarrays were described by Kononen et al. for the first time. Since then, this high-throughput method has been adopted by many investigators across the world. Sections from tissue microarrays are used for cost-effective and rapid molecular profiling of tissue samples from large patient cohorts, in particular in the field of cancer research. Different applications of tissue microarrays are reviewed and an in-depth discussion provided of the importance of tissue microarray design, quality control and the technical aspects of its construction. Advantages and disadvantages of this modern technology are described. Drawing on our own experiences, potential problems are highlighted and practical solutions are offered.

Keywords: construction, design, quality control, tissue microarray

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PII: S1756-2317(09)00024-3

doi:10.1016/j.mpdhp.2009.01.010

Diagnostic Histopathology
Volume 15, Issue 3 , Pages 142-150, March 2009

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