Diagnostic Histopathology
Volume 18, Issue 2 , Pages 53-63, February 2012

Molecular diagnostics in lymphoma: why, when and how to apply

Tim C Diss PhD is a Clinical Scientist at the Histopathology Department, University College London Hospital and Department of Pathology, University College London, UK. Conflicts of interest: none declared

Thierry J Molina MD PhD is a Pathologist at the Service de Pathologie, AP-HP, Hôpitaux Universitaires Paris Centre, Université Paris Descartes, Paris, France. Conflicts of interest: none declared

Jose Cabeçadas MD is a Pathologist at the Departamento de Diagnóstico Laboratorial IPOLFG – Serviço de Anatomia Patológica, Lisbon, Portugal. Conflicts of interest: none declared

Anton W Langerak PhD is a Medical Immunologist at Erasmus Medical Centre, Rotterdam, The Netherlands. Conflicts of interest: none declared

published online 19 December 2011.

Abstract 

Current strategies for diagnosing lymphomas include molecular methodologies next to histomorphological assessment supplemented by immunohistochemistry. Especially PCR-based clonality analysis and detection of chromosome aberrations by FISH can support the diagnosis and classification in difficult cases. We have to ensure that these existing molecular methodologies are used to their full potential. Yet, such analyses are not required in every situation. Ongoing optimization, dissemination of (new) protocols, standardized interpretation, and education are essential elements to ensure best practice for patient care. Here we discuss why, when, and how to apply these molecular diagnostic methods in the field of lymphoma.

Keywords: clonality, FISH, Ig/TCR, leukemia, lymphoma, PCR, translocation

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PII: S1756-2317(11)00193-9

doi:10.1016/j.mpdhp.2011.11.005

Diagnostic Histopathology
Volume 18, Issue 2 , Pages 53-63, February 2012

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